Keywords
low molecular weight compounds
silver-109 nanoparticles
LDI MS
LDI MSI
Abstract
Mycotoxins are toxic secondary metabolites produced by specific fungi, known for their structural diversity and high chemical stability. Their synthesis is influenced by environmental factors, leading to regional variability. Mycotoxins, such as citrinin, ochratoxin A, patulin, roquefortine C, and sterigmatocystin, pose significant health risks to humans and animals. Common detection methods include chromatography and ELISA, with liquid chromatography-tandem mass spectrometry (LC-MS/MS) being the gold standard for its sensitivity and selectivity. Alternative methods, like laser desorption/ionization mass spectrometry (LDI-MS), offer unique advantages but face challenges like high background noise and low ionization efficiency for nonpolar compounds. This study explores the detection and quantification of mycotoxins using silver-109 nanoparticles on a steel plate, produced via a novel pulsed fiber laser method with a 2D galvanometer scanner. Comparative analysis of manual LDI-MS and semi-automatic LDI-MSI for sterigmatocystin quantification highlights their effectiveness, providing a promising alternative for accurate mycotoxin analysis.
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